Special Sets|
For more details, please write us.
Strains of Chlamydomonas eugametos and C. moewusii, mostly from C.S. Gowans and Ralph Lewin. These include auxotrophic mutants, flagellar mutants, a few antibiotic resistant strains. Wild-type strains; all the standard lab strains of C. reinhardtii, plus natural isolates from Canada, Minnesota, Pennsylvania, North Carolina and Florida. Motility mutants. In addition to the well-studied pf, fla, oda, etc. mutants, we have some old mutants from McVittie and elsewhere showing impaired motility but otherwise uncharacterized. gliding motility mutants. A set of 20 mutants defective in gliding motility, isolated by Keith Kozminski when he was in the Rosenbaum lab Strains with altered phototaxisUV-sensitive mutants Mutants deficient in chloroplast ribosomes Antibiotic resistant mutants: these include chloroplast mutants resistant to streptomycin, spectinomycin, kanamycin, erythromycin, and a few other antibiotics; also some nuclear mutants. Some have been well-characterized, others haven't. Temperature-sensitive lethals Fluoroacetate-resistant, collection of 38 mutants from N.E. Tolbert and Barbara Wilson RFLP mapping strains from Silflow and Lefebvre Herbicide resistant mutants: These include chloroplast mutants resistant to DCMU, atrazine, bromacil, etc.; some nuclear mutants with similar phenotypes; mutants resistant to chlorsulfuron or imazaquin; and a collection of mutants resistant to some experimental herbicides that were being developed by Ciba-Geigy in the 1980s; at least two of these are sulfonyl ureas. Please write for details. Acetate-requiring strains. We have many acetate-requiring strains, some with nuclear mutations and others chloroplast, that have not been well-characterized. If anyone would like a sampler, e.g. for a student project, we can send a set. RbcS2 strains. From Arminio Boschetti, a collection of transformants of the Rubisco knockout mutant rbcS-T60-3 in which various site-directed mutations were made in the transit peptide VHLR strains. From Britta Forster, mutants resistant to very high light intensities. osm strains. Strains with defects in osmotic regulation, from Peter Luykx; should be grown on TAP medium supplemented with lactose or sucrose |