Spectinomycin resistance mutations in chloroplast 16S ribosomal RNA
Mutations in the 16S rRNA conferring spectinomycin resistance map to a conserved stem region in the 3' portion of the RNA. All the mutants whose sequence is known destroy an AatII site in this region.
Allele: spr-u-1-6-2
(A>G at nt1123, equivalent to E. coli nt1191; this mutation confers high level spectinomycin resistance under all growth conditions)
Strains:
CC-110 spr-u-1-6-2 mt+
CC-145 nr-u-2-1 spr-u-1-6-2 sr-u-2-60 mt-
CC-146 nr1-1 spr-u-1-6-2 mt-
CC-207 nr1-5 spr-u-1-6-2 mt+
CC-218 er-u-37 spr-u-1-6-2 mt+
CC-219 spr-u-1-6-2 sr-u-2-60 mt+
CC-225 spr-u-1-6-2 sr-u-2-60 mt-
CC-227 er-u-37 spr-u-1-6-2 sr-u-2-60 mt+
CC-328 er-u-37 spr-u-1-6-2 mt+
CC-412 ac17 act2 er-u-37 nic13 spr-u-1-6-2 sr-u-2-60 mt-
CC-1069 er-u-37 nic13 spr-u-1-6-2 sr-u-2-60 mt+
CC-1070 er-u-37 nic13 spr-u-1-6-2 sr-u-2-60 mt-
CC-1087 nr1-5 spr-u-1-6-2 thi1 mt+
CC-2011 FUD18 sr-u-2-60 spr-u-1-6-2 mt+
CC-2284 dr-u-2 spr-u-1-6-2 mt+
CC-2285 dr-u-2 spr-u-1-6-2 mt-
CC-2658 arg7 spr-u-1-6-2 mt+
CC-2661 ac29a nic7 spr-u-1-6-2 mt-
CC-2676 dr-u-2 spr-u-1-6-2 mt-
CC-2800 spr-u-1-6-2 sr-u-2-60 mt+, disrupted atpB
CC-2801 spr-u-1-6-2 sr-u-2-60 mt+, disrupted atpB
CC-2802 spr-u-1-6-2 sr-u-2-60 mt+, disrupted atpB
CC-2803 spr-u-1-6-2 sr-u-2-60 mt+, disrupted rbcL
CC-2804 spr-u-1-6-2 sr-u-2-60 mt+, disrupted rbcL
CC-2805 spr-u-1-6-2 sr-u-2-60 mt+, disrupted rbcL
CC-2807 spr-u-1-6-2 mt+
CC-2831 psbA-NP spr-u-1-6-2 mt+
CC-2832 psbA-NP spr-u-1-6-2 mt+
CC-2833 psbA-NP spr-u-1-6-2 mt+
CC-2834 psbA-NP spr-u-1-6-2 mt+
CC-2835 psbA-NP spr-u-1-6-2 mt+
CC-2962 petA-A15* spr-u-1-6-2 sr-u-2-60 mt+
CC-2963 petA-A12E spr-u-1-6-2 sr-u-2-60 mt+
CC-2964 petA-A15E spr-u-1-6-2 sr-u-2-60 mt+
CC-2965 petA-V16D spr-u-1-6-2 sr-u-2-60 mt+
CC-2966 petA-M20E spr-u-1-6-2 sr-u-2-60 mt+
CC-2967 petA-M20K spr-u-1-6-2 sr-u-2-60 mt+
CC-2968 petA-HCFS spr-u-1-6-2 sr-u-2-60 mt+
CC-2969 petA-HC-(delta) spr-u-1-6-2 sr-u-2-60 mt+
Allele: spr-u-1-H-4
(Identical mutation to spr-u-1-6-2, A>G at nt1123, equivalent to E. coli nt1191; this mutation confers high level spectinomycin resistance under all growth conditions)
Strains:
Allele: spr-u-sp-23
(A>C at nt1123, equivalent to E. coli nt1191; this mutation confers high level spectinomycin resistance under all growth conditions)
Strains:
CC-217 spr-u-sp-23 (nit+) mt+
CC-223 spr-u-sp-23 sr-u-sm2 (nit+) mt+
CC-224 spr-u-sp-23 sr-u-sm2 (nit+) mt-
CC-271 er-u-RS-1 spr-u-sp-23 (nit+) mt+
CC-379 act1 spr-u sr-u-sm6 (nit+) mt+
CC-506 act1 er-u olr-u-1 spr-u (nit+) mt+
CC-1153 er? me1 spr-u-sp-23 sr-u-sm2 mt-
CC-1312 mat-1 spr-u-sp-23 sr-u-sm2 mt-
CC-2480 ac-u-c-2-29 spr-u-sp-23 sr-u-sm2
Allele: spr-u-1-27-3
(G>A at nt1125, equivalent to E. coli nt1193; confers relatively low levels of spectinomycin resistance. Cells of this mutant grown on minimal medium on agar are killed by 100 micrograms/ml spectinomycin but will grow on acetate at this concentration)
Strains:
CC-104 spr-u-1-27-3 mt+
CC-105 spr-u-1-27-3 mt+
CC-220 er-u-1a spr-u-1-27-3 mt+
CC-327 nr-u-2-1 spr-u-1-27-3 mt-
CC-346 spr-u-1-27-3 sr-u-2-23 mt+
CC-421 ac29a nic7 spr-u-1-27-3 mt-
CC-426 cw15 nic1 spr-u-1-27-3 mt-
CC-434 ac-u-b-1-5 spr-u-1-27-3 mt+
CC-435 ac-u-b-1-10 spr-u-1-27-3 mt+
CC-436 ac-u-a-1-15 spr-u-1-27-3 mt-
CC-438 ac-u-i-2-25 spr-u-1-27-3 mt+
CC-698 ac-u-g-2-3 spr-u-1-27-3 mt+
CC-706 spr-u-1-27-3 mt-
CC-707 ac-u-c-2-9 spr-u-1-27-3 mt-
CC-743 ac-u-a-1-15 spr-u-1-27-3 mt+
CC-814 ac-u-d-2-12 spr-u-1-27-3 mt-
CC-1096 ac-u-e-1-24 spr-u-1-27-3 mt-
CC-1130 ac-u-d-2-12 spr-u-1-27-3 mt+
CC-1133 ac-u-g-2-3 spr-u-1-27-3 mt-
CC-1135 ac-u-e-1-24 spr-u-1-27-3 mt+
CC-1933 mat3-1 spr-u-1-27-3 mt+
CC-1995 mat3-2 spr-u-1-27-3 mt+
CC-1996 mat3-3 spr-u-1-27-3 mt+
CC-2351 ac-u-a-1-15R spr-u-1-27-3 mt+
CC-2467 ac-u-c-2-9 er-u-11 spr-u-1-27-3 mt+
CC-2478 sd-u-2-24 spr-u-1-27-3 mt-
CC-2646 mat3-2 spr-u-1-27-3 mt+
CC-2959 agl1 arg2 msr1 spr-u-1-27-3 mt-
CC-3095 er-u-37 spr-u-1-27-3 mt+
Allele: spr-u-1-5-2
(Not sequenced, but mutation destroys AatII site in 16S rRNA gene)
Strains:
For more information on Chlamydomonas mutants, see the Allele category in ChlamyDB.
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