Chlamydomonas Genetics Center

Neamine/kanamycin resistance mutations in chloroplast 16S ribosomal RNA

These mutations, mapping to the extreme 3' end of the 16S rRNA, confer resistance to both neamine and kanamycin.

Allele: nr-u-2-1
(A>G at nt1340, equivalent to E. coli nt1408)

CC-86 nr-u-2-1 mt+

CC-87 nr-u-2-1 mt-

CC-130 msr1 nr-u-2-1 sr-u-2-60 mt+

CC-137 er-u-37 nr-u-2-1 spr-u-1-H-4 mt-

CC-138 er-u-37 nr-u-2-1 spr-u-1-H-4 sr-u-2-60 mt+

CC-139 er-u-37 nr-u-2-1 spr-u-1-H-4 sr-u-2-60 mt-

CC-145 nr-u-2-1 spr-u-1-6-2 sr-u-2-60 mt-

CC-245 nr-u-2-1 spr-u-1-H-4 mt+

CC-307 nr-u-2-1 sr-u-sm-3a mt+

CC-355 er-u-37 nr-u-2-1 mt+

CC-422 ac29a nic7 nr-u-2-1 mt-

CC-1067 arg2 nr-u-2-1 mt+

CC-1068 arg2 nr-u-2-1 mt-

Allele: kr-u-24-2
(C>U at nt1341, equivalent to E. coli nt1409)
The kr-u-24-2 mutant resembles nr-u-2-1 in its resistance to neamine or kanamycin on agar plates, but the chloroplast ribosomes isolated from this mutant are not as resistant to these antibiotics as those of nr-u-2-1 when assayed in poly-U-directed amino acid incorporation in vitro.
Strains:

CC-187 kr-u-24-2 mt+

Allele: kr-u-35-3
(not sequenced, but phenotypically indistinguishable from kr-u-24-2)
Strains:

For more information on Chlamydomonas mutants, see the Allele category in ChlamyDB.

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