CC-86 nr-u-2-1 mt+

Locus: 16S rRNA, chloroplast genome

Source: Product from a cross of wild-type mt+ (N.W. Gillham's strain, equivalent to CC-125) to nr-u-2-1 sr-u-2-60 mt-.

Comment: This is a useful chloroplast marker, mapping to the 3' end of the chloroplast 16S ribosomal RNA. Although isolated initially as a neamine-resistant strain, it is also resistant to kanamycin, a more readily available antibiotic. CC-86 was used in many crosses in the Boynton-Gillham laboratory in the 1970s.