CC-43 ac20 mt-

Locus: AC20, linkage group I

Source: Product 5.6.1 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)

Comment: This strain was used by Boynton et al., J. Cell Sci. 10, 267-305 (1972) in their study of mutants deficient in chloroplast ribosomes, and defines the ac20 mutation in terms of chloroplast ribosome content (deficient in both ribosomal subunits).Like other mutants deficient in chloroplast ribosomes, ac20 is easiest to score by slow growth on minimal medium at cold temperature (15-17 degrees). Considered purely as a genetic marker, ac20 is not the best choice for this region of linkage group I. However, CC-43 appears to be a stable strain with the correct ac20 phenotype.