CC-155 ac20 cr1 mt+

Locus: AC20, linkage group I
Locus: CR1, linkage group XII/XIII

Source: Product 5.6.2 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)

Comment: This strain was used by Boynton et al., J. Cell Sci. 10, 267-305 (1972) in their study of mutants deficient in chloroplast ribosomes. This strain is a good choice when maximal deficiency in chloroplast protein synthesis is desired. Even so, this strain does not have a stringent acetate requirement, and does grow slightly on minimal medium. Scoring is easier at cool temperatures, e.g. 15 degrees.