CC-104 spr-u-1-27-3 mt+

Locus: 16S rRNA, linkage group chloroplast

Source: CC-104 was derived from a cross of CC-105 spr-u-1-27-3 mt+ to an ac20 cr1 strain. A tetratype tetrad was selected; this is the product that carries the wild type alleles of the ac20 and cr1 mutations.

Comment: Because this strain was used frequently in chloroplast genetics studies in the Boynton-Gillham laboratory, it was chosen from among several spr-u-1-27-3 strains as the representative mt+ stock for that mutation in the core collection. Spectinomycin resistance is easy to score in this strain on acetate medium, but it does not grow well on spectinomycin in minimal medium.