Culture Collection

The Chlamydomonas Center culture collection supplies Chlamydomonas strains, kits for teaching and science projects, and molecular resources. The collection is supported by a grant from the National Science Foundation, and by fees paid by users of our cultures.

Core Collection

The most frequently requested strains.

We are frequently asked what strain was used for genomic sequencing. This was CC-503 cw92 mt+, a cell-wall deficient strain, and was used in order to get high-quality DNA. Most of the EST libraries sequenced at Stanford were prepared in the wild-type strain CC-1690 21 gr mt+. Both CC-503 and CC-1690 derive from the same original field isolate, made in Massachusetts in 1945, but they have been separate since at least the mid-1950s. S1 D2, a strain used for some ESTs and comparative sequencing, is a field isolate from Minnesota, dating from the 1980s.

Special Sets

These are sets of strains with various phenotypes, including acetate requiring, non-motile, herbicide resistant, fluoroacetate resistant, temperature-sensitive, and other phenotypes. Many of them are poorly characterized and deserve more attention.

Archival Strains

Mostly historical strains that document publications, or that have been replaced with more recent isolates.

Project Kits

Strains and materials for high school science projects and laboratory exercises at the high school or college level

Molecular Mapping Kit

Primers and polymorphic strain for mapping genes in preparation for mapped-based cloning

Libraries

BAC and cDNA libraries created for the Chlamydomonas Genome Project

Plasmids

Individual cDNA and genomic clones. Currently the collection is heavy on chloroplast genes, less complete for nuclear. We welcome contributions of additional plasmids, especially of useful constructs for transformation and gene expression.

Media

We also sell Hutner's trace elements

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