Methods

We'd like to add more files to this page - if you have suggestions, or files you can contribute, please write us: chlamy@duke.edu

Recipes for culture media

TAP, HS, Sager and Granick, Hutner's trace

Mating and tetrad separation of Chlamydomonas reinhardtii for genetic analysis

video by Xingshan Jiang and David Stern, published on-line, Journal of Visualized Experiments, August 12, 2009; PMID: 19684568

Laboratory protocols for molecular genetics of Chlamydomonas

EMBO Practical Course: Molecular Genetics of Chlamydomonas, Laboratory Protocols, 4-9 (2006)

archived version of Craig's Chlamy Corner

many useful methods for molecular biology

Ble Marker for Nuclear Transformation

Saul Purton's page

Hygromycin Resistance Vectors for Nuclear Transformation

from Peter Berthold and Wolfgang Mages

Antibodies

For information on commercially available antibodies to Chlamydomonas proteins, please contact Dr. Joanna Porankiewicz-Asplund

Quick and Easy Genomic DNA Prep for PCR Analysis

from Steve Pollock

Cleaning Up Contaminated Cultures

discussion from bionet.chlamydomonas, several contributors

Isolating Chlamydomonas from Soil Samples

from Cliff Zeyl

Freezing Chlamydomonas Cells

from Dick Sayre; method involves methanol as cryoprotectant and storage in liquid nitrogen, the most effective protocol as yet developed

Encouraging Chlamydomonas Strains to Mate

from Bob Hodson

Mating Type Tests in Multiwell Plates

from Andy Wang

Preparation of Gamete Autolysin

discussion from bionet.chlamydomonas, several contributors

Preparation of Gamete Autolysin

improved protocol, from Sabine Waffenschmidt May 2006

Chlamydomonas Cells Sticking to Glass

compiled by Tjibbe-Chris Kuipers for bionet.chlamydomonas; several contributors

Tris as N Source for Chlamydomonas?

compiled by Xavier Reboud for bionet.chlamydomonas; several contributors

Fixation Method for Mitotic Cells

from Peter Luykx

Minipreps of DNA from Chlamydomonas Cultures

compiled by Hank Latorella for bionet.chlamydomonas; several contributors

Transformation of Chlamydomonas with Glass Beads

from Karen Kindle

Improving Transformation Efficiencies

compiled by Roel Funke for bionet.chlamydomonas; several contributors

Problems Cutting Genomic DNA

from Cliff Zeyl

Antisense and Gene Expression

from Karen Kindle

PCR of Chlamydomonas Nuclear DNA

from Barbara Randolph-Anderson

Extraction of RNA from Chlamydomonas

from Takako Kato

Sequencing GC-Rich Nuclear DNA from Chlamydomonas

compiled by Emma Berta Gutierrez for bionet.chlamydomonas; several contributors

Transposon Insertions

compiled by Kris Niyogi for bionet.chlamydomonas; several contributors