Methods

Most of these files were culled from past posts to bionet.chlamydomonas. We'd like to add more - if you have suggestions, or files you can contribute, please write us: chlamy@duke.edu

Recipes for culture media

TAP, HS, Sager and Granick, Hutner's trace

Hygromycin Resistance Marker for Nuclear Transformation

from Peter Berthold and Wolfgang Mages

Quick and Easy Genomic DNA Prep for PCR Analysis

from Steve Pollock

Cleaning Up Contaminated Cultures

discussion from bionet.chlamydomonas, several contributors

Isolating Chlamydomonas from Soil Samples

from Cliff Zeyl

Freezing Chlamydomonas Cells

from Dick Sayre; method involves methanol as cryoprotectant and storage in liquid nitrogen, the most effective protocol as yet developed

Encouraging Chlamydomonas Strains to Mate

from Bob Hodson

Mating Type Tests in Multiwell Plates

from Andy Wang

Preparation of Gamete Autolysin

discussion from bionet.chlamydomonas, several contributors

Preparation of Gamete Autolysin

improved protocol, from Sabine Waffenschmidt May 2006

Chlamydomonas Cells Sticking to Glass

compiled by Tjibbe-Chris Kuipers for bionet.chlamydomonas; several contributors

Tris as N Source for Chlamydomonas?

compiled by Xavier Reboud for bionet.chlamydomonas; several contributors

Fixation Method for Mitotic Cells

from Peter Luykx

Minipreps of DNA from Chlamydomonas Cultures

compiled by Hank Latorella for bionet.chlamydomonas; several contributors

Transformation of Chlamydomonas with Glass Beads

from Karen Kindle

Improving Transformation Efficiencies

compiled by Roel Funke for bionet.chlamydomonas; several contributors

Problems Cutting Genomic DNA

from Cliff Zeyl

Antisense and Gene Expression

from Karen Kindle

PCR of Chlamydomonas Nuclear DNA

from Barbara Randolph-Anderson

Extraction of RNA from Chlamydomonas

from Takako Kato

Sequencing GC-Rich Nuclear DNA from Chlamydomonas

compiled by Emma Berta Gutierrez for bionet.chlamydomonas; several contributors

Transposon Insertions

compiled by Kris Niyogi for bionet.chlamydomonas; several contributors

Ble Marker for Nuclear Transformation

Saul Purton's page

Craig's Chlamy Corner includes methods files used in the Silflow/Lefebvre lab at the University of Minnesota