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Methods
Most of these files were culled from past posts to bionet.chlamydomonas. We'd like to add more - if you have suggestions, or files you can contribute, please write us: chlamy@duke.edu
- Recipes for culture media
- TAP, HS, Sager and Granick, Hutner's trace
- Hygromycin Resistance Marker for Nuclear Transformation
- from Peter Berthold and Wolfgang Mages
- Quick and Easy Genomic DNA Prep for PCR Analysis
- from Steve Pollock
- Cleaning Up Contaminated Cultures
- discussion from bionet.chlamydomonas, several contributors
- Isolating Chlamydomonas from Soil Samples
- from Cliff Zeyl
- Freezing Chlamydomonas Cells
- from Dick Sayre; method involves methanol as cryoprotectant and storage in liquid nitrogen, the most effective protocol as yet developed
- Encouraging Chlamydomonas Strains to Mate
- from Bob Hodson
- Mating Type Tests in Multiwell Plates
- from Andy Wang
- Preparation of Gamete Autolysin
- discussion from bionet.chlamydomonas, several contributors
- Preparation of Gamete Autolysin
- improved protocol, from Sabine Waffenschmidt May 2006
- Chlamydomonas Cells Sticking to Glass
- compiled by Tjibbe-Chris Kuipers for bionet.chlamydomonas; several contributors
- Tris as N Source for Chlamydomonas?
- compiled by Xavier Reboud for bionet.chlamydomonas; several contributors
- Fixation Method for Mitotic Cells
- from Peter Luykx
- Minipreps of DNA from Chlamydomonas Cultures
- compiled by Hank Latorella for bionet.chlamydomonas; several contributors
- Transformation of Chlamydomonas with Glass Beads
- from Karen Kindle
- Improving Transformation Efficiencies
- compiled by Roel Funke for bionet.chlamydomonas; several contributors
- Problems Cutting Genomic DNA
- from Cliff Zeyl
- Antisense and Gene Expression
- from Karen Kindle
- PCR of Chlamydomonas Nuclear DNA
- from Barbara Randolph-Anderson
- Extraction of RNA from Chlamydomonas
- from Takako Kato
- Sequencing GC-Rich Nuclear DNA from Chlamydomonas
- compiled by Emma Berta Gutierrez for bionet.chlamydomonas; several contributors
- Transposon Insertions
- compiled by Kris Niyogi for bionet.chlamydomonas; several contributors
- Ble Marker for Nuclear Transformation
- Saul Purton's page
- Craig's Chlamy Corner includes methods files used in the Silflow/Lefebvre lab at the University of Minnesota
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