from Gorman, D.S., and R.P. Levine (1965) Proc. Natl. Acad. Sci. USA 54, 1665-1669.
This is probably the most widely-used medium at present for experimental work.
Make the following stock solutions:
1. TAP salts
| NH4Cl | 15.0 g | |
| MgSO4 . 7H2O | 4.0 g | |
| CaCl2 . 2H2O | 2.0 g | |
| water to 1 liter |
2. phosphate solution
| K2HPO4 | 28.8 g | |
| KH2PO4 | 14.4 g | |
| water to 100 ml |
3. Hutner's trace elements (follow this link)
To make the final medium, mix the following:
2.42 g Tris
25 ml solution #1 (salts)
0.375 ml solution #2 (phosphate)
1.0 ml solution #3 (trace elements)
1.0 ml glacial acetic acid
water to 1 liter
For solid medium, add 15 g agar per liter
Autoclave.
For Tris-minimal medium omit the acetic acid and titrate the final solution to pH 7.0 with HCl
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